The high-copy plasmids listed here contain mutated versions of this origin. * The pMB1 origin of replication is closely related to that of ColE1 and falls in the same incompatibility group. Origins of replication and copy numbers of various plasmids and cosmids A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture. A way to determine experimentally if the copy number of your plasmid is high or low is to perform a miniprep. This table can also be found online at the QIAGEN Plasmid Resource Center in the section ' Growth of bacterial cultures Plasmid Copy Number'. ![]() Transfection, cloning, sequencing, capillary sequencing, etc.įind out which origin of replication your plasmid contains, and look at the table below for classification into high-copy or low-copy types.
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